The past few decades have witnessed the rapid progress in the development of responsive probes for luminescence bioassay, biomedical research, and pre-/clinical investigations. The development of reliable bioanalytical probes for selective and sensitive detection of particular biomolecule in biological systems plays key roles in luminescence bioassay and is essential for better understanding the roles of the biomolecule in their native contexts. In this context, a large number of fluorescent responsive probes have been developed for biomolecules detection in vitro and in vivo. Nevertheless, it remains challenging to use these probes for biomolecules detection in the samples with high background autofluorescence signals. In this presentation, I will introduce our recent contributions to the development of responsive probes for background-free luminescence bioassay and imaging. Specifically, responsive metal complex probes with long-lifetime emission for time-gated luminescence (TGL) analysis and imaging of biomolecules in vitro and in vivo will be discussed.

Background-free luminescence bioassay and imaging with responsive probes

ACS Spring Meeting 2021

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