Prebiotic compounds play a major role in proliferating human gut microbiome thus helps to prevent severe gastric problems and facilitate boosting immune system. Being an under-utilised crop, Prachyrhyzus erosus (Yam bean) tubers have been utilised as a source of prebiotic substance, inulin. The inulin was extracted using conventional hot water method (yield of 9% w/w) that showed around 48.86% higher prebiotic potential over Lactobacillus fermentum compared to glucose. To understand the interaction between oligomers and the target site, in-silico analysis was conducted that showed inulin with monomer units of 3 and 7 had better binding efficiency towards inulinase enzyme than compared to entire polymer chain. This docking study was validated under laboratory condition by enzymatic hydrolysis followed by activity of hydrolysates over target microorganism. About 14.28% and 28.57% increase in activity was observed for oligomers having monomer units of 3 and 7 compared to inulin polymer respectively. Further the inulin was used to encapsulate β-carotene through emulsification by homogenisation process. Confocal laser scanning microscopic image and XRD spectrum confirmed formation of encapsulated product. Encapsulation efficiency and loading capacity were achieved about 66% and 1.2% respectively. Higher antioxidant activity was observed for the developed product compared to their ingredients. Cumulative release of β-carotene from the formulation was around 49.65% and 75.23% under simulated gastric and intestinal fluid conditions respectively. Fitting release data into korsmeyer peppas model, similar kind of release pattern (non-fickian diffusion, 0.5&lt;n&lt;1) were found for both the conditions. This study helps to visualise the utilization of inulin in order to obtain its prebiotic potential at its best possible way.

Exploration of Prachyrhyzus erosus (Yam bean) tubers as potential source of inulin, a potent prebiotic for development of functional products: An approach towards nutraceutical advancement

ACS Spring Meeting 2021

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