Multidimensional Liquid Chromatography (LC) is a key development and application area for complex sample analysis. During the recent years much progress has been made in enhancing compatibility between the two separation dimensions. As such, several separation modes, such as (multiple) heart-cutting, high-resolution sampling and comprehensive 2D-LC are available to meet the researcher’s needs. In this talk, examples will be provided showing the power of 2D-LC for very fast separations, and for measurements at highest peak capacities. For target component analysis in epoxy resins, the 2D-LC method employs UHP-SEC analysis with tetrahydrofuran (THF) in 1D and UHPLC with acetonitrile/water mobile phase gradient in 2D. Active solvent modulation (ASM) is utilized to enhance compatibility between both separation modes. 80 µL of strong solvent THF are injected onto a 50x3 mm core-shell C18 column without sample breakthrough occurring. A total run time of less than 3 min could be achieved. For analysis of a very complex industrial sample containing aromatic amines, comprehensive 2D-LC (LCxLC) was employed. By coupling six pentafluorophenyl columns in 1D and using a short C18 column in 2D, a peak capacity of higher than 11,000 could be obtained within 20 h analysis time. More than 900 individual peaks could be observed with this methodology.

Recent advances in two-dimensional liquid chromatography – from ultra-fast to ultra-high resolution

ACS Spring Meeting 2021

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