The construction of polymer based 3-D architectures with nanoscale precision is an essential facet of macromolecular science to create nano-objects that exhibit complex and programmable behaviour. However, the synthesis of polymer nanostructures with customizable shape profiles, contour and surface chemistry at molecular resolution is extremely challenging due to thermodynamic factors that control shape persistency and stability. Although the scientific need is straightforward, there are currently very few tools that allow to tailor 3-D nanoarchitectures on demand.<br/>To address this, we developed a technology that allows us to exploit the structural interfaces of DNA and proteins, such that polymers or polymerization reactions can be guided by the monodispersity and sequence of the respective biomolecules. One strategy involves the use of DNA-origami nanotechnology where initiator molecules or catalysts can be positioned in different geometrical patterns to produce polymers of the desired shape. We also demonstrate that the 3-D engineering of cross-sectional areas of the nano-object is also possible, allowing independent customization of the internal volume and the outer surface.<br/>The second method involves the use of proteins, where the emphasis is on sequence controlled chemistry. Taking advantage of the absolute amino acid positions along the protein backbone, both regioselective and chemoselective chemistry can be used in conjunction to tailor anisotropic polymer-protein brushes with site specific modifications. By developing these two strategic approaches, we aim to feature the potential of biomolecules in the synthesis of colloidal nano-objects with molecular resolution.

Synthesis of precise polymeric nano-objects guided by biomolecular interfaces

ACS Spring Meeting 2021

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