Efavirenz, also known as Sustiva, is a medication prescribed for the prevention and treatment of HIV/AIDS. The metabolism of efavirenz rapidly produces pharmaceutically inactive analog molecules. Two common metabolites are 7-hydroxy efavirenz (7-OH) and 8-hydroxy efavirenz (8-OH). HPLC-MS/MS provides the standard method of analysis for both efavirenz and the metabolic analogs but requires significant resources for the operation and maintenance of a triple-quadrupole based mass analyzer. In this work, standard solutions of efavirenz, as well as 7-OH and 8-OH are investigated using atmospheric pressure Electrospray Ionization Ion Mobility Spectrometry (ESI-IMS). ESI-IMS may act as a detector for HPLC separation and its ability to separate isomers rapidly with parts-per-billion detection limits may allow IMS to operate in place of MS/MS instrumentation with significantly lower operating costs. The prior demonstration of negative-mode mass spectral detection of these compounds provided initial conditions under which to investigate IMS as a separation mechanism. Standalone IMS results are compared with IMS-mass spectrometry data in order to deduce the viability of IMS as a separation and detection method for these compounds. Solution condition dependence of the ESI process are compared with literature results for HPLC-MS/MS in order to optimize the separation.

Ion mobility spectrometry detection of efavirenz and its metabolic analogs

ACS Spring Meeting 2021

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