Reactive oxygen species (ROS) are byproducts of metabolic reactions involving oxygen that are involved in many natural and anthropogenic processes. However, overproduction of ROS is known to cause oxidative stress, cell damage, and disease. In addition, ROS are highly reactive and short-lived, making them important, but challenging, analytical targets.<br/>Asymmetric ionic materials (AIMs), which include ionic liquids and solid-phase ionic materials, are organic salts whose ions are mismatched in size and shape. While ionic liquids have been widely studied, solid-phase AIMs, including nanoscale materials comprised of asymmetric salts, are lacking investigation in spite of their potential for use in sensor development and adaptive physical and spectral properties. Probes based on nanoscale asymmetric salts offer a promising avenue for tunable fluorescent nanoprobes for ROS, as reported in the literature, but more research is needed to identify and evaluate these types of sensors.<br/>The work reported here uses time-dependent multichannel UV-Vis absorbance and frequency-domain dynamic fluorescence measurements to evaluate and compare two types of solid-phase AIMs by monitoring the evolution of their spectral properties during their synthesis. Anionic exchange reactions will be used to associate both the target (imidazolium with 2,7’-dichlorofluorescein) and reference AIM (1,1’-diethyl-2,2’-carbocyanine with bis(trifluoromethanesulfonyl)imide) in a biphasic solution of DCM and water. The resulting multivariate spectral data sets will be analyzed by curve resolution to detect changes in number and properties of the components of the reaction mixture, facilitating optimization of solid-phase AIM synthesis reaction conditions, detection of reaction completion, and correlation of spectral shifts to probe structural changes.

Fluorescence reaction progress monitoring of solid-phase asymmetric ionic nanoscale probe synthesis

ACS Spring Meeting 2021

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