Supported Lipid Bilayers (SLBs) are a form of model biological membrane which are composed of a bilayer of phospholipids supported on a non-metal oxide surface such as silicon dioxide. SLBs typically form when a buffered solution containing lipid vesicles is flowed over the surface. Although the formation of SLBs has been known for more than thirty years, there is still not a clear understanding of the mechanism by which they form. By fitting data acquired using a Quartz Crystal Microbalance (QCM) to a modified Michaelis-Menten Langmuir (MML) type kinetic model we have been able to extract data such as rate constants for the adsorption and rupture of the vesicles. We have made modifications to the model to also account for what happens when we interrupt the flow of lipids over the surface during SLB formation.

Development of a kinetic model for the formation of supported lipid bilayers

ACS Spring Meeting 2021

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