Variable domain heavy/heavy chain antibody, lacking in light chains (VHH) antibodies have high affinity, stability, productivity, and processability for antigens. Antibody based drugs are typically stored in a refrigerated or frozen state, but this may lead to protein denaturation from changes in temperature or repeated freezing and thawing. Therefore, it is essential to evaluate the structural stability in order to maintain quality control. For this work samples of VHH antibodies which has high specificity against the receptor binding domain of SARS-CoV-2 were used. VHH samples were submitted to different thermal conditions, from room temperature to -10°C, and intermittent thermal cycles from - 25°C to room temperature, the latter was repeated three times. Secondary structure was estimated using Fourier transform infrared (FTIR) spectroscopy. FTIR spectra were collected using a single bounce ATR crystal and a multi bounce ATR crystal for small amount sample with low concentration. VHH antibody used in this experiment does not significantly change its secondary structure during cooling, freezing and thawing, exhibiting high structural stability

Effect of thermal cycling on secondary structure estimation of VHH antibodies

ACS Spring Meeting 2021

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.