The metabolism of retinoids influences the homeostasis of the skin and represents an important target of therapies in dermatology. Short chain dehydrogenase/reductase family 16C member 5 (SDR16C5) is a member of the SDR enzyme family that converts retinol into retinaldehyde and retinaldehyde into retinoic acid. The aim of this study was to determine the expression pattern of SDR16C5 in human skin and skin appendages. Human primary epidermal keratinocytes were induced to differentiate in vitro. SDR16C5 mRNA and protein levels were determined by quantitative RT-PCR and western blot analysis. The expression pattern of SDR16C5 in human skin was investigated by immunohistochemistry. Gene expression analysis in human organs showed predominant expression of SDR16C5 in the skin. SDR16C5 mRNA and protein strongly increased in abundance during terminal differentiation of keratinocytes in monolayer culture and in organotypic skin models. In human skin, SDR16C5 was detected in the granular layer of the epidermis. In addition, SDR16C5 was expressed in sebaceous glands. The results of this study suggest that SDR16C5 is a keratinocyte differentiation-dependent regulator of cutaneous retinoid metabolism.

ESDR141 - Short chain dehydrogenase/reductase family 16C member 5 (SDR16C5) is a keratinocyte differentiation-dependent regulator of cutaneous retinoid metabolism

50th Annual Meeting of the European Society for Dermatological Research. VIRTUAL MEETING