Psoriasis is a common immune-mediated skin disease involving a wide range of epithelial and immune cells. The development of an adequate and complex model remains a major challenge in the understanding of psoriasis’ molecular basis. In this study, we investigated the impact of activated T cells on the molecular and the inflammatory microenvironment characteristic of psoriasis when included in our tissue-engineered psoriatic skin model. To this end, activated T cells were seeded into healthy (HS) and psoriatic (PS) skin models. PS inflammatory microenvironment exhibited significantly higher expression of various chemokines and cytokines such as IL-17A, IFNƴ, CCL2, CXCL10, IL-1ra, IL-6 and CXCL8 compared to HS. Moreover, anti-IL17A treatment restored the expression of Ki67, S100A7, elafin and involucrin of PS. These results highlighted the role of pathological keratinocytes in the initiation of cutaneous inflammation. Overall, our study demonstrated that this model reflects an appropriate psoriatic inflammatory environment and is responsive to anti-IL17A treatment, making it a valuable tool for drug development.

ESDRLB002 - Investigation of communication between T cells and pathological keratinocytes in a tissue engineered model of psoriatic skin

50th Annual Meeting of the European Society for Dermatological Research. VIRTUAL MEETING