Fast-scan cyclic voltammetry (FSCV) is a robust technique for electrochemical analysis of neurotransmitters. FSCV relies on the ease and effectiveness of electrode calibration for quanitfication of experimental changes. Flow injection analysis is most commonly used to mimic dynamic changes in neurotransmitters that occur in vivo using macrofluidic flow cells. In this work, we design and characterize a novel microfluidic flow cell with integrated FSCV electrodes, fabricated with lithography for easy to use, reproducible in vitro calibration of voltammetric serotonin measurements in the nanomolar to micromolar range. We examined the effect of the dimension, flow parameters, and material properties of the flow cell on the conformance of the current injection profile to an ideal step-wise injection while characterizing the performance of the nanofabricated electrodes. Following this investigation, we evaluated the analytical performance of our design via comparison with the traditional macrofluidic flow injection system. We thus present a microfluidic flow cell design as a solution to limitations encountered by our lab during electrochemical calibration to improve the reliability of measurements collected with FSCV.

The design of a microfluidic flow cell with integrated electrodes for in-vitro calibration of serotonin measurements using fast scan cyclic voltammetry

ACS Fall 2021

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