A live cell is a complex container. Understanding the physical chemistry in terms of dynamics and spectra at a selected intracellular component (organelle) is a challenging task. Recently, we have made significant progress in this direction using confocal microscopy. The smallest size of the focused spot in a confocal microscope is 0.2 mm (200 nm). This is nearly one hundred times smaller than the size of a live cell. This offers spatial resolution to study a live cell. In this talk, we discuss how one can image intracellular components/organelles, record fluorescence spectra and decay, ascertain local polarity and viscosity, and monitor the dynamics of solvation, proton transfer, red-ox and other phenomena inside a cell. We will also highlight behaviour of intrinsically disordered protein in a crowded polymer hydrogel.

Time resolved microscopy: Live cell and crowded systems

ACS Fall 2021

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.