Malondialdehyde (MDA) is an important biomarker of oxidative stress. It is also a product of lipid peroxidation induced by free radicals in the human body. This research study shows a reliable new method for detection of MDA in vitro condition, based on the formation of a colored and emissive product of reaction with para-methoxy aniline (PMA). In this preliminary work, a detection method for a yellowish, stable MDA-PMA derivative with minimal time and volume of sample by Gas Chromatography Mass Spectrometry (GC-MS) was introduced. Injections (2 µl) were performed by hand, in the splitless mode, using an initial temperature of 200°C and a purging time of 1.00 min. Separation was achieved on a 5% phenylmethyl siloxane capillary column with a column head pressure of 5.29 psi; a purge flow of 25 ml/min and a purge time of 1.00 min. The initial temperature was held at 50°C and followed by a rate of 10°C to a 180°C (held for 1 min) and a rate of 20 to 280°C (held for 1 min). The solvent delay was 3.60 min, the threshold was 150, and the electron energy was measured at 69.922 eV. Finally, as can be seen in Figure 1 (a), and (b), the MDA-PMA adduct was identified in the SCAN mode at a temperature of 300°C (held for 5 minutes to facilitate cleaning of the column). The total analysis time was about 20 minutes, and the equilibration time was held at 0.50 minutes. The mass-to-charge ratio range spanned was 50-550.Overall, the application of ever-improving GC-MS techniques to detect MDA by a new reagent (PMA) in human samples at yet unexplored frontiers in disease detection and daily routines. Thus, this method predicts to receive an advantage from the reasonable separation factor by eliminating the extraction step within the sample pretreatment.

Characterization of malondialdehyde through gas chromatography-mass spectrometry by a new reagent

ACS Fall 2021

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