Glutamic acid decarboxylases (GAD) 65 and 67 catalyze the decarboxylation of glutamic acid to γ-aminobutyric acid (GABA), an inhibitory neurotransmitter. GABA regulates cognition, behavior, and physiological responses to stress. We hypothesized that GAD is a biomarker for anxiety in the brain and GAD expression may reflect if a subject experienced social isolation-induced anxiety. The effect of gender on GAD expression and social isolation was also studied. To test these hypotheses, Long-Evans rats were raised in group-housed and socially isolated conditions. Elevated plus maze and open-field tests were used to detect and measure anxiety. Western blots were performed to quantify GAD expression in prefrontal cortices, hippocampi, and cerebella. Results obtained through western blots and behavioral tests will be presented.

Glutamic acid decarboxylase isoforms: Potential biomarkers for anxiety and social isolation

ACS Fall 2021

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