Local fatty acid composition of blood vessels is important in development of atherosclerosis. The present study compared the fatty acid composition of non-blocked left internal mammary artery (LIMA) vessels which are used in Coronary Artery Bypass Graft surgery and atherosclerotic endarteroctemy plaque (ER) samples. Patients (n=9) undergoing elective CABG were enrolled in the study. Small biopsy of segments (1-2 g) of left LIMA and ER of patients were collected during the surgery. Fatty acid (FA) profile of tissue samples was analyzed by Gas Chromatography Mass Spectroscopy.The major saturated fatty acid (SFA) reported in both samples was palmitic acid (LIMA: 36.04 ± 13.57 % and ER: 41.19 ± 12.29 %). The percentage of lauric acid (LIMA: 6.49 ± 3.40% and ER:2.91 ± 1.81 %) and myristic acid (LIMA: 9.18 ±4.67 and ER: 5.27 ± 3.05) were significantly high in LIMA compared to ER. A comparison of FA profile revealed higher SFA (ER: 56.06± 7.6 and LIMA: 54.63± 12.8 ) and low unsaturated FA (LIMA: 9.18 ± 4.67 and ER: 5.27 ± 3.05) content in ER than LIMA. High percentage of arachidonic acid (AA) - ω -6 (ER: 4.23± 2.92 and LIMA: 0.41 ± 0.20%, p=0.013) and docosahexaenoic acid (DHA) - ω -3 (LIMA: 0.41 ± 0.29 and ER: 0.96 ± 0.83) were observed in ER samples. A significantly high ratio between AA -ω -6 to DHA - ω -3 poly unsaturated fatty acids (LIMA: 1.8 ± 0.57 and ER: 4.5 ± 1.7, p=0.04) were observed in the tissue samples of ER compared to LIMA. The high percentage of pro inflammatory AA and high ratio of AA : DHA composition in ER is indicated the inflammatory role of AA in atherosclerosis.

Omega–6 fatty acid good or bad? A reveal from atherosclerotic plaque samples

ACS Fall 2021

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.