Health functionalities of kimchi have been extensively demonstrated and well-known to originate from active component from kimchi as well as kimchi itself. Non-alcoholic fatty liver disease (NAFLD) is a kind of fatty liver disease and occurs without alcohol or drug intake by obesity, diabetes, and hypertension. Endoplasmic reticulum (ER) stress also causes several diseases, including obesity, diabetes, and NAFLD. Various attempts have been demonstrated using food source to prevent ER stress-induced NAFLD. Herein, our study aimed to investigate the effect of kimchi active components and kimchi itself in NAFLD. Therefore, we investigated the effect of kimchi activie components and kimchi in ER-stress-induced HepG2 cells and its related-mechanism. To induce ER stress, tunicamycin (TM) or palmitic acid (PA) was treated in hepG2 cells. Cell viability, intracellular triglyceride (TG) content, and ER stress, inflammation, and lipogenesis-related genes level were measured. In addition, the mechanism study on hepatic fat accumulation was performed. Firstly, alliin, capsaicin, and gingerol significantly decreased ER stress, inflammation, lipogenesis genes expression, and intracellular TG content in TM- or PA-induced hepG2 cells with low cytotoxicity (p&lt;0.05). In mechanism results, alliin, capsaicin, and gingerol significantly inhibited LXR agonist (T0901317)-induced lipogenesis and promoted AMP-activated protein kinase (AMPK) agonist (AICAR)-induced AMPK activation (p&lt;0.05). Secondly, kimchi also significantly reduced ER stress, inflammation, lipogenesis genes expression, and intracellular TG content in TM- or PA-induced hepG2 cells with low cytotoxicity (p&lt;0.05). In consistent with kimchi active component results, kimchi is significantly inhibited T0901317-induced lipogenesis and promoted AICAR-induced AMPK activation (p&lt;0.05). Taken all together, kimchi have powerful potential to prevent NAFLD by inhibition of hepatic lipogenesis by regulating AMPK activation. To confirm this study, improvement effect of kimchi and kimchi active component in ER stress-induced animal model will conduct in further study.

Improvement effect of kimchi and kimchi active components on ER stress-induced non-alcoholic fatty liver disease via AMPK activation

ACS Fall 2021

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