<p style="text-align: justify">In three-dimensional cell culture, key parameters such as cell concentration and material concentration may affect cell survival rate, proliferation and differentiation ability and other functional expression, which has very important practical significance, It has great research value in analytical chemistry, microarray, drug screening, tissue culture and so on. In this paper, the principle of active mixing is introduced for dynamic mixers. The moving parts are biocompatible mixers. Different components of alginate gel are mixed quickly in the mixing chamber, and finally the homogenized material is extruded through the replacement needle installed at the outlet of the mixing chamber. The feeding system is a push rod injection pump, and the linear motion of the injection pump is transformed into the liquid flow rate of the gel solution through a single chip microcomputer, and the flow feed is precisely controlled. In addition, by changing the flow rate ratio of the two components solution and the rapid mixing of the micro mixer, the real-time concentration change of the mixed material at the outlet can be realized. In order to optimize the preparation of heterogeneous gel microspheres, the working process of the micromixer was modeled and simulated, which could provide better guidance for the design of subsequent micromixers and the preparation of gel microspheres. Finally, the animal cells in three-dimensional culture of sodium alginate Gel Microspheres and their application is discussed. The fibroblasts of L929 mice were mixed in the printed sodium alginate heterogel gel and cultured in vitro, the results showed that the cells survived well in the three-dimensional sodium alginate hydrogel.</p>

Three-Dimensional Cell Culture With Alginate Hetero Gel Microspheres

Paper Proceedings: https://asmedigitalcollection.asme.org/MSEC/MSEC2021/volume/85062

2021 International Manufacturing Science and Engineering Conference (MSEC2021)

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