<p>The directional arrangement and extension of the cells is of great significance in the field of tissue engineering. Great efforts have been made to study the effects of simple micro- or nano-structures on cell behavior, but whether the hybrid micro/nano structures have the same effect on cells is also worthy of further study. In this paper, a method of fabricating hybrid micro/nano structures by combining laser marking technology with metal assisted chemical etching (MACE) is introduced to investigate the growth behavior of SHSY5Y cells onto hybrid micro/nano structures. It was found that the cells on the silicon micro-pit arrays (SiMP arrays, unetched substrate) arrange orderly along the edge of the micro-pits, stretch and connect with each other, while the cells on the hybrid silicon micro-pit and silicon nano-pillar arrays (hybrid SiMP/SiNP arrays, etched substrate) are also arranged in an orderly manner with a relatively short cell stretch, but displayed a preference for independent growth. In addition, almost 90% of cells on the nano-pillars (NPs) area, but only 10% of cells on the micro-pits (MPs) area on hybrid SiMP/SiNP arrays. The results suggest that hybrid SiMP/SiNP arrays have a trap and elongation effect on cells, and the independent growth behavior of cells on this substrate is of great meaningful in the field of single neuron research.</p>

Growth Behavior of SHSY5Y Cells on Hybrid Micro-pit and Nano-pillar Arrays

Paper Proceedings: https://asmedigitalcollection.asme.org/IDETC-CIE/IDETC-CIE2021/volume/85369

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