Most molecular tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, the virus responsible for COVID-19) are limited to the laboratory and thus have significant lag times (&gt;24 hours) to provide a result, preventing their widespread adoption in point-of-care settings. I will describe the design and development of a paper-based diagnostic test that detects the nucleic acids from SARS-CoV-2 to produce a color change that is visible to the naked eye. This test uses loop-mediated isothermal amplification (LAMP) and detects the virus in saliva samples. Using image analysis, the paper-based device detects the virus within 60 minutes, has an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/µL of contrived saliva. The paper-based test is easily configurable to detect multiple targets by increasing the number of reaction zones in the device. It has the potential to detect a variety of pathogens simply by changing the LAMP primer sets.

Paper-based point-of-care diagnostics for COVID-19

ACS Spring 2022

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