Reactive nitrogen species (RNS) are reactive molecules derived from nitric oxide (●NO) and oxygen species such as superoxide (O2●−). While they play in numerous biological processes, excessive concentration of RNS triggers a reaction with reactive oxygen species and damage cells. Peroxynitrite (ONOO−) is one of the reactive nitrogen species. In a biological system, ONOO− act as a physiological mediator but its abnormal regulation is associated with diseases including cardiovascular function, neurodegeneration, cancer, and asthma. Complex and potent reactivity of ONOO− emphasize the importance to develop sensitive and direct detection methods, which will offer new opportunities for biomedical research and the development of therapeutics. Reaction-based chemiluminescent probes were acknowledged for high sensitivity and selectivity in detecting ONOO−, but precise quantitative measurement of the concentration of ONOO− remains a challenge. My presentation focuses on developing tools and methods to measure the concentration of ONOO− in vitro and in living cells. Using a chemiluminescent probe synthesized in our lab as a study model, I am working with a stopped-flow instrument to measure response between trigger moiety and ONOO− under pseudo-first order conditions. Rate constants were calculated and obtained, and further studies with chemiluminescent probes and different triggers were conducted.

Precise quantitative measurement of reactive nitrogen species

ACS Spring 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.