Recombinant Adeno-Associated Viruses (rAAVs) deliver therapeutic DNA for gene therapy. However, rAAV manufacturing is imperfect, producing mostly defective capsids without the complete therapeutic gene. Here, we developed nanofluidic resonators for characterizing rAAV by measuring their mass. We juxtapose our approach with techniques ranging from ddPCR and Analytical Ultracentrifugation to Static Light Scattering. With our rAAV characterization approach, we envision to enable real-time quality control in continuous rAAV manufacturing.

Measuring the DNA content of adeno-associated viruses using nanofluidic resonators

ACS Spring 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.