Adenosine triphosphate (ATP) is an important biochemical molecule, due to its role in many biological pathways. It’s hydrolysis energizes most metabolic pathways. Understanding ATP’s biological role requires studies on the stability of the molecule at varying pH conditions. To do this, aqueous solutions of ATP at various pH have been analyzed using UV-visible spectroscopy. Its patterns were examined to provide information about the stability of ATP. Clear distinctions were observed in the spectra for solutions below pH 5 and above pH 5. These differences in spectra above and below pH 5 were compared to complementary analyses for ADP and AMP solutions.

Qualitative measurement of stability of ATP at varying pH using UV-Vis spectroscopy

ACS Spring 2022

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