Glutamate is the primary excitatory neurotransmitter in the brain and influences all forms of behavior, including consciousness, motor control, and memory. Upon arrival of an action potential, glutamate is released into the synaptic cleft. During the release event, the concentration of glutamate in the synaptic cleft reaches micromolar level, and then rapidly returns to nanomolar concentration due to the uptake by neurons and glia. The local extrasynaptic glutamate concentration is sufficient to activate extrasynaptic metabotropic (mGluR) and ionotropic (NMDA) glutamate receptors. Dysregulation of glutamate is implicated in receptor-mediated excitotoxicity and is associated with multiple diseases, such as Alzheimer's, Huntington’s, and Parkinson' s diseases.The ability to accurately measure extracellular glutamate concentration is critical for uncovering its role in metabolic processes, such as cellular redox potential and neurometabolic coupling between synaptic activity, glial metabolism, and blood flow. Knowledge of these regulation mechanisms is important for understanding the physiology of excitatory neuroplasticity and its role in neuropsychiatric diseases. Here we present the design of the optical glutamate sensor, composed of the glutamate recognition unit and the fluorescent reporters. The synthesis and properties of the sensor are discussed.

Development of glutamate sensor for <i>in vivo</i> applications

ACS Spring 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

Development of glutamate sensor for in vivo applications