Accurately and precisely measuring lipoprotein subclasses is necessary for the early detection of cardiovascular disease (CVD). Clinical methods incorporate labor and time intensive immunochromatographic techniques to quantify lipoprotein subclasses, and several methods use a derivative calculation to estimate lipoprotein fractions indirectly. During recent years, these methods alone show difficulties in achieving the precision required for distinguishing individuals with and without CVD. Our methodology individually separates the lipoprotein fractions by density and isolates them for further characterizations including dynamic light scattering sizing and chromatographic techniques. There are a multitude of applications downstream that can be utilized once the individual classes are separated by density. This technique is efficient, reproducible, facile, and rapid. Density Gradient Ultracentrifugal (DGU) lipoprotein profiling is a powerful technique that can examine patients at risk of cardiovascular disease and result in early detection and better prevention of CVD.

Density gradient ultracentrifugal lipoprotein profiling

ACS Spring 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.