Cervical cancer is one of the deadly cancers for women that caused by Human papillomaviruses. Human papillomavirus virus-like particles (HPV VLPs) have been shown to be effective in preventing cervical cancer when used as a prophylactic vaccine. However, little attention has been paid to the process of L1 expression and self-assembly of VLPs in vivo. In this study, pH, temperature and methanol optimized in shake flask level to optimize L1 expression and self-assembly into HPV VLPs. HPV 16 L1 (pPICZ vector) was expressed in <i>Pichia pastoris</i> X-33, with zeocin selection marker and successfully self-assembled into HPV 16 VLPs. The results demonstrate that there is a strong correlation between pH, temperature and methanol concentration with the expression of HPV L1 as well as VLP formation. To conclude, if the pH, temperature methanol concentration is too low or too high, the levels of HPV 16 L1 VLPs can easily decrease during the shake flask processing.

Process optimization for the production of HPV-16 L1 VLP in <i>Pichia Pastoris</i>

ACS Spring 2022

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Process optimization for the production of HPV-16 L1 VLP in Pichia Pastoris