To solve crimes, forensic scientists test evidence including trace fiber, plant material, and pollen. In many cases, the origin of botanical matter has had significant legal value. In this presentation, we report upon differentiating <i>Quercus alba</i> white oak trees by DNA testing using primers that probe single nucleotide polymorphisms (SNPs) via PCR and high-resolution melt analysis. The plant DNA was extracted using the Qiagen DNeasy plant mini kit and quantified using a Nanodrop UV-Vis spectrometer. The PCR and melts were conducted using a Qiagen Rotor-Gene Q real-time PCR instrument.

DNA typing to differentiate white oak trees using real-time PCR and high-resolution melt analysis

ACS Spring 2022

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