Peptides have returned to the forefront of drug discovery efforts as unbiased screening technologies have improved and the rules defining passive cell permeability have been clarified.  As a result, the demand for synthetic peptide libraries has increased significantly, particularly during secondary screening as lead compound programs progress.  At these early discovery phases though, highly pure peptide samples may not be necessary when evaluating these compound libraries in assays.  With this in mind and given the improvements in automated peptide library synthesis strategies, a solid phase extraction methodology may be appropriate to parallelize peptide library cleanup and significantly reduce library purification time.<br/>Herein we present data demonstrating that sorbent selection, specifically the sorbent parameters themselves, impacts the predictability of elution profile when using solid phase extraction to improve the purity of synthetic peptide libraries across a wide range of therapeutically relevant peptides.

Choosing the appropriate solid phase extraction sorbent for peptide library clean up

ACS Spring 2022

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