4-Hydroxynonenal (4-HNE) is one of the secondary products of lipid peroxidation and has been studied in the greatest detail, due to its high reactivity and consequently, its role in a wide range of biological processes. During oxidative stress, reactive oxygen species involved in a free radical chain mechanism can induce the production of lipid peroxyl radicals, hydroperoxides and a few toxic secondary byproducts such as malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE). the latter byproduct is the target analyte for our proposed sensor. In this project, the goal is to design a turn on fluorescent sensor that could quantitate 4-HNE levels with high selectivity in serum. To this aim, we plan to develop a Thiol-based sensor that enables us to take advantage of Thiol-Michael addition reaction with our target analyte, 4-HNE. Dansyl Chloride was selected as the Fluorophore for this sensor. The synthesis of the probe will be discussed, and spectroscopic and binding data will be presented.

Development of a novel fluorescent sensor for detection and quantification of 4-hydroxynonenal

ACS Spring 2022

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