During last few decades Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) have been widely used for treating numerous inflammatory diseases. One of the pathways that NSAID generates anti-inflammatory activity via inhibiting cyclooxygenase enzyme, that converts arachidonic acid to prostaglandins. Natural products as COX2 inhibitors are promising compared to synthetic ones due to their minimal side effects. In this study we have investigated the potential inhibitory activities of COX1 and COX2 by a dietary supplement S7, a proprietary polyphenol-rich fruit, vegetable and herbs-based material previously reported to reduce reactive oxygen species and to increase level of bioavailable NO in humans. S7 blend includes ingredients: green tea extract (46.5%), green coffee bean extract (46.5%), turmeric extract (5%), whole blueberry powder (0.5%), whole tart cherry powder (0.5%), broccoli powder (0.5) and kale powder (0.5%). The results suggested that S7 blend showed significant inhibition of COX1 and COX2 activities in concentration dependent manner up to 79 and 87 % respectively with the concentration range 1.13-22.27 µg/mL. Among its ingredients green tea extract showed strongest inhibition up to 68 and 97 % respectively in the tested concentrations. Green coffee bean extract showed moderate inhibition (33%) in the same concentration. Other ingredients did not show significant inhibition against COX1 and COX2 activities in the assay. Selective inhibition of COX2 over COX1 were found to be better by minimizing gastrointestinal effects associated with COX-1. This study of screening of various food product as potential inhibitors against COX1 and CO2 is expected to guide us to develop effective natural dietary plant products that provide anti-inflammatory properties with limited or no side effects.

Selective dietary plant extract as inhibitors of cyclooxygenase -1 and cyclooxygenase-2 activities

ACS Spring 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.