Anti-obesity action of nonviable probiotic lactic acid bacteria (PLAB) may be attributed to bacterial cellular components recognized by host cells. Anti-inflammation and anti-obesity properties of surface layer proteins (SLPs) that are cellular components isolated from kefir PLAB were determined in macrophage RAW 264.7 cells and obese mice. Kefir SLPs significantly decreased secretion of IL-6 and production of NF-kB p65 protein by LPS-stimulated RAW 264.7 cells in a dose-response manner. C57BL/6J mice were fed a high-fat (HF) diet with oral administration of either saline (CON) or kefir SLPs for six weeks. SLPs significantly reduced HF-induced weight gain and adipose tissue weight, plasma triglyceride concentrations, and insulin resistance. Profiling of adipocyte gene expression showed that anti-obesity effect was significantly related to expression of genes associated with adipogenesis, autophagy, and inflammatory/immune response, and fatty acid oxidation. Taken together, SLPs are a novel bioactive component in kefir PLABs to target obesity and obesity-related disorders.

Bioactive components in parabiotic kefir lactic acid bacteria and obesity

ACS Spring 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.