Immunoassays have been extensively used in many fields, including tacking of food fraud, due to their overwhelming overall advantages in high sensitivity, excellent selectivity, simplicity, fast speed, high throughput, low cost, safety, and general applicability. The key of an excellent immunoassay is the availability of antibodies with high affinity, specificity, and batch-to-batch consistency. Single domain antibody, also termed nanobody or VHH, is an antibody with a single variable domain derived from heavy-chain only antibodies in camelids or cartilaginous fish. This recombinant antibody has received increasing interest owing to their small size, monoclonal nature, genetic manipulability, high thermostability, superior solubility, ease of clone storage and expression in diverse expression platforms, and cost effectiveness for both discovery and continuous production. In this section, we will present the work on the development of nanobodies and the nanobody based immunoassays and further explore their potential applications for tracing of food fraud.

Exploration of nanobody based immunoassays for the tracing of food fraud

ACS Spring 2022

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