Introduction: There is a lot of literature about the application of keratinocytes in deep second degree burns but there is still a lack about the effect of dressings on keratinocytes. Polylactic membranes have demonstrated usefulness in the treatment of superficial and partial thickness burns because of pain reduction, ease of application, reduced workload and good clinical results. This paper presents the results of 103 patients treated with sprayed keratinocytes and covered with polylactic membranes.
Methods: Retrospective quality control in 103 patients from 2003 to 2018, in which partial-thickness burns were treated with sprayed keratinocytes and covered with poly lactic membranes. From 2003 to 2015 evaluation of burn depth was done on clinical aspects, from 2016 to 2018 laser Doppler imaging was used. People were included, when the time of healing was expected to be longer than 15 days.
Results: There were 67 male and 36 female patients with an average TBSA of 17.8% ±14.8 median: 14. Average second-degree burn was 8.52 %(±9.8), average 3rd° burn was 9.47% plus ±12. 2. Age was in average 39 years (median 42). No significant difference could be found between male and female in age and TBSA distribution . Average abbreviated burn severity index was 5.97 , interestingly in women significantly higher. Nearly all patients had significant comorbidities. Source of injury was explosion (18), flame (55), scald burn(17), contact burn (2), electricity (2), suicide attempt(6) and others (2). Operation was started on average 4 days (±3 ) after the injury (median 3.0). Sprayed keratinocytes were applied in average 12 days after the 1stoperation (±7.6). Using laser Doppler imaging the time for keratinocytes application was reduced to 7.6 days . Three different cell types were applied to twelve different regions. Healing time (time to 99% healing) was on average 8. 33 days (median: 7). The total treatment time to healing was shorter in the non-cultured cell suspension treated patients due to the lacking necessity of pre- culturing cells. In 6 patients re- transplantation had to be done. The last control was performed on average 96 days (±368) after spraying. Scar was visible in 4 , pigment changes happened in 9, hypertrophic scarring in 5 patients, Infections were suspected or confirmed in 6.
Conclusions: Polylactic membranes proved to be an adequate dressing for sprayed keratinocytes in deep 2nd° burns . There was no need for frequent dressing changes. Infections in other areas of the body did not spread on the wounds covered with polylactic membranes. Other than predicted 91% of the wounds were healed within 14 days;. 12 cases treated with not cultured cells and polylactic membranes were completely healed within 15 days.

**Introduction:** There is a lot of literature about the application of keratinocytes in deep second degree burns but there is still a lack about the effect of dressings on keratinocytes. Polylactic membranes have demonstrated usefulness in the treatment of superficial and partial thickness burns because of pain reduction, ease of application, reduced workload and good clinical results. This paper presents the results of 103 patients treated with sprayed keratinocytes and covered with polylactic membranes.
**Methods:** Retrospective quality control in 103 patients from 2003 to 2018, in which partial-thickness burns were treated with sprayed keratinocytes and covered with poly lactic membranes. From 2003 to 2015 evaluation of burn depth was done on clinical aspects, from 2016 to 2018 laser Doppler imaging was used. People were included, when the time of healing was expected to be longer than 15 days.
**Results:** There were 67 male and 36 female patients with an average TBSA of 17.8% ±14.8 median: 14. Average second-degree burn was 8.52 %(±9.8), average 3rd° burn was 9.47% plus ±12. 2. Age was in average 39 years (median 42). No significant difference could be found between male and female in age and TBSA distribution . Average abbreviated burn severity index was 5.97 , interestingly in women significantly higher. Nearly all patients had significant comorbidities. Source of injury was explosion (18), flame (55), scald burn(17), contact burn (2), electricity (2), suicide attempt(6) and others (2). Operation was started on average 4 days (±3 ) after the injury (median 3.0). Sprayed keratinocytes were applied in average 12 days after the 1stoperation (±7.6). Using laser Doppler imaging the time for keratinocytes application was reduced to 7.6 days . Three different cell types were applied to twelve different regions. Healing time (time to 99% healing) was on average 8. 33 days (median: 7). The total treatment time to healing was shorter in the non-cultured cell suspension treated patients due to the lacking necessity of pre- culturing cells. In 6 patients re- transplantation had to be done. The last control was performed on average 96 days (±368) after spraying. Scar was visible in 4 , pigment changes happened in 9, hypertrophic scarring in 5 patients, Infections were suspected or confirmed in 6.
**Conclusions:** Polylactic membranes proved to be an adequate dressing for sprayed keratinocytes in deep 2nd° burns . There was no need for frequent dressing changes. Infections in other areas of the body did not spread on the wounds covered with polylactic membranes. Other than predicted 91% of the wounds were healed within 14 days;. 12 cases treated with not cultured cells and polylactic membranes were completely healed within 15 days.

P.148 Use of polylactic membranes as a dressing for sprayed autologous skin cells in deep dermal burn wounds.

Dear friends and colleagues,

We will be pleased to welcome you in Turin (Italy) for the 19th European Burns Association´s Congress in 2022. Italy is honored to hold the meeting of the European Burns Association for the second time, after Verona in 1995. Turin is easily reachable from everywhere in Europe, either travelling by car, train or choosing flight connections. During the meeting, held in the renewed industrial center of the main Italian historical automotive factory (i.e. Lingotto-FIAT), we will meet international burn specialists, focusing on standards of care, progresses in burn treatment, strategies to face disaster situations, with a special attention to education of younger colleagues. However, in your spare time, you might enjoy the sweet days of Italian late summer, simply wandering in the green parks and gardens along the two rivers which cross the city, or you might decide to discover the numerous art galleries, churches, palaces, libraries, walking across elegant squares and large boulevards, passing through centuries of art and history; finally, you may choose to immerse yourself in some of the most exciting museum experiences, such as the Cinema Museum and the Turin’s Egyptian museum.

Looking forward to seeing you all in Turin for the 19th European Association´s Congress in 2022!
Maurizio Stella, Congress President, Turin Burn Centre