The aim of this work was to evaluate the performances of the recently developed in vivo imaging device Line-Field Confocal Optical Coherence Tomography (LC-OCT, DAMAE Medical, which has a better resolution that classical OCT) compared to the Confocal Laser Scanning Microscopy (CLSM, Vivascope 1500, Mavig) from a qualitative point of view and assessment of the epidermal thickness according to age. We conducted a mono-centric open-label study. A total of 17 subjects divided into three groups (3–6 months, 5–7 years and 20–25 years) were recruited. Acquisitions have been performed on volar forearm. CLSM allows horizontal plane visualization. LC-OCT allows horizontal, vertical, and three-dimensional visualization with a better resolution in the vertical axis, a wider field of view and a faster image acquisition compared to CLSM. Full epidermis thickness was calculated as the mean value between the surface and the dermo-epidermal junction on a vertical reconstruction for CLSM and on a vertical acquisition for LC-OCT. Acquisitions with both devices allow a visualization of the epidermis at cellular level. Results obtained with both devices give a slight decrease of full epidermis thickness according to age. This result is in accordance with a previous study using classical OCT but differs from those obtained using CLSM without reconstruction of the vertical plane of the epidermis. This pilot study demonstrated the ability of both devices for the in vivo skin imaging on different age groups and their coherence. LC-OCT offers a new way for skin analysis and specially for pediatric applications. Larger studies are required to further interpret the discrepancies on epidermis thickness variation described by previous works.

ESDR221 - Line-Field Confocal Optical Coherence Tomography compared with Confocal Laser Scanning Microscopy: a pilot study on three different age groups

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