Recent advances have been made toward developing three-dimensional (3D) in vitro human skin models, including skin characteristics in terms of epidermal and dermal structures, barrier functions, cell migration, and cell-to-cell interaction in the 3D microenvironment. However, current 3D in vitro human skin models have been a limitation that monitors long-term real-time for validating in vitro, at least, with the co-culture of two main types of skin cells. To overcome these limitations, we investigated whether decellularized bioscaffolds of non-human origin provide a naturally derived microenvironment for skin-associated cells to be adhesion, migration, growth, and differentiation their great merits. The decellularized bioscaffolds of non-human origins were made from pig tissue with muscle/fat based on combination methods of chemical and physical tools. Hematoxylin and eosin (H&E), scanning electron microscopy, Hoechst 3342 staining, and genomic material contents demonstrate the complete decellularization of bioscaffolds. A novel co-culture system of skin microenvironment was fabricated in an epidermal layer and dermal layer seeded with a clip-mold and multiple cell-based collagen solution. To prove our hypothesis, CDy6 mitotic tracker probe-stained fluorescence imaging, and qRT-PCR analysis show that 3D in vitro decellularized bioscaffold-mediated skin models are suitable conditions for the maintenance and survival rate of compatible different cell lines. In conclusion, our findings can be useful for a new intelligent and more efficient safety assessment, based on in vitro testing into a predictive safety assessment of biomaterials.

ESDR242 - A Novel Co-culture System of Multiple Cell Types for Mimicking Skin Microenvironment

The scientific meeting of the European Society for Dermatological Research (ESDR) is held yearly, usually in September. With an international attendance of more than 1000 skin scientists from around the globe, the four-day event includes short oral presentations from peer-reviewed abstracts, keynote lectures from international experts, sponsored symposia, satellite programs, educational events as well as an important focus on posters, networking and building collaborations amongst scientists. For more information about this year’s meeting visit our website.