In the three past decades, tissue engineering allowed to develop skin models with characteristics close to ex vivo skin, particularly at the level of the epidermis. The main function of the epidermis is to protect the body against environmental damage (UVs, pollution, microbial pathogens…) and to prevent water loss. Keratinocytes, the major cellular component of the epidermis, proliferate and differentiate to form in fine a physical barrier sustaining the epidermal barrier function. This cutaneous impermeability is ensured by the formation of a well-defined stratum corneum constituted by corneocytes and a lipidic matrix. The presence of specific lipids and proteins is essential and required to form a good barrier function. The characterization by immunohistochemistry targeting specific proteins of the upper layers of the epidermis, allowed to confirm the correct differentiation of the bioengineered epidermis. Besides, the detection of specific lipids (cholesterol, ceramides and fatty acid) by LC/MS allowed to characterize the pattern of lipid expression. This combination of skin bioengineering and the ability to chemically characterize the keratinocyte lipids provides a promising platform for studying the effects of topically or systemically applied biofunctional ingredients or chemical substances with modulatory potential in vitro.

ESDR254 - Characterization of keratinocyte lipid composition of bioengineered human epidermis

The scientific meeting of the European Society for Dermatological Research (ESDR) is held yearly, usually in September. With an international attendance of more than 1000 skin scientists from around the globe, the four-day event includes short oral presentations from peer-reviewed abstracts, keynote lectures from international experts, sponsored symposia, satellite programs, educational events as well as an important focus on posters, networking and building collaborations amongst scientists. For more information about this year’s meeting visit our website.