Acne vulgaris is a disorder affecting the pilosebaceous unit resulting in both inflammatory and non-inflammatory clinical lesions.Cutibacterium acnes (C. acnes) of a specific lineage, plays a key role in inflammatory acne by producing several virulence factors thereby increasing their inflammatory capacity. The C. acnes mutated CAMP1 factor display strong TLR-2 binding property in highly inflammatory C. acnes strains. CAMP1 peptide array analysis of 116 15-mer peptides sequences with an overlap of 11 amino acids, were immobilized onto glass plate and showed that seven peptides (A14, A15, B1, B2, B3, C1 and C3) were specifically recognized by TLR-2 and located on the same side of the in silico 3D-CAMP1 structure. Among these seven peptides, 2 peptides (A14 and B2) were able to compete the interaction CAMP1-TLR-2 in vitro. Moreover, 3 peptides (A14, B1 and B2) were able to dose-dependently decrease the C. acnes-induced CX-CL8 production in keratinocytes as well as to decrease at the transcriptional and translational levels of the ex vivo production of GM-CSF, TNF-α, IL-1β, and IL-10 in human skin explants stimulated with recombinant (r)CAMP1 protein. RNA sequencing analysis of skin explants pretreated with B2 and stimulated with rCAMP1, identified 10 genes, IL6, TNF, CXCL1, CXCL2, CXCL3, CXCL8, IL-1β, CCL2, CCL4 and CSF2, harboring strong differential expression. All these genes were validated by qRT-PCR. We characterized a new C. acnes CAMP1-derived peptide which could be used as a potential TLR-2 modulator to reduce TLR-2-induced inflammation.

ESDR357 - Cutibacterium acnes CAMP factor 1-related peptides as Toll-Like Receptor-2 modulators

The scientific meeting of the European Society for Dermatological Research (ESDR) is held yearly, usually in September. With an international attendance of more than 1000 skin scientists from around the globe, the four-day event includes short oral presentations from peer-reviewed abstracts, keynote lectures from international experts, sponsored symposia, satellite programs, educational events as well as an important focus on posters, networking and building collaborations amongst scientists. For more information about this year’s meeting visit our website.