Atopic dermatitis (AD) is a heterogeneous disease and characterized by dysregulated immune response of diverse immune cell subtypes, such as Th2, Th22, and Th17. Heterogeneity of AD can be reasoned by varying contribution of underlying immune subsets, as observed by altered cytokine milieus in the skin. These variations result in changes of protein expression in keratinocytes, including antimicrobial peptides (AMPs). We hypothesize that antimicrobial peptide expression may correlate with the underlying immune subtypes and, therefore, can be used as potential biomarkers to predict treatment outcome. In this study, we used non-invasive tape stripping to collect stratum corneum samples and determine the concentration of AMPs on the skin of patients with AD under systemic or topical treatment. To quantify AMP concentration we developed a new magnetic bead-based multiplex immunoassay, allowing simultaneous detection of six different AMPs (HBD-2, HBD-3, RNase 7, Dermcidin, S100A7 and S100A8/S100A9 Heterodimer). We found that expression of AMPs differs in lesional skin compared to samples of healthy controls, depending on current efficacy of treatment and body site. Observed variations in AMP concentration point towards varying contribution of underlying immune subsets. Therefore, our multiplex assay represents a useful tool for simultaneous detection of AMPs in the skin.

ESDR412 - Development of Bead-Based Multiplex Immunoassay for Antimicrobial Peptide Detection in Stratum Corneum of Atopic Dermatitis Skin

The scientific meeting of the European Society for Dermatological Research (ESDR) is held yearly, usually in September. With an international attendance of more than 1000 skin scientists from around the globe, the four-day event includes short oral presentations from peer-reviewed abstracts, keynote lectures from international experts, sponsored symposia, satellite programs, educational events as well as an important focus on posters, networking and building collaborations amongst scientists. For more information about this year’s meeting visit our website.