Skin pigmentation depends on cellular processes including biogenesis, transport, maturation, and transfer of melanosomes to keratinocytes. However, the mechanisms associated with the fine spatial and temporal controls to ensure adequate pigmentation remain unclear. Here, we show that a component of the cytoplasmic dynein complex, Dynlt3, is required for the transport, acidity regulation and efficient transfer of melanosomes to keratinocytes. In Mus musculus melanocytes with decreased levels of Dynlt3, pigmented melanosomes undergo a more directional movement, leading to their peripheral localization in the cell. Stage IV melanosomes are more acidic than normal, but still highly pigmented, resulting in less efficient melanosome transfer. Finally, the level of Dynlt3 is dependent on β-catenin activity, revealing a function of the Wnt/β-catenin signaling pathway during melanocyte and skin pigmentation, coupling the transport, positioning, and acidity of melanosomes necessary for their transfer.

ESDR516 - Dynein is involved in the movement, distribution, acidity, and transfer of melanosomes

The scientific meeting of the European Society for Dermatological Research (ESDR) is held yearly, usually in September. With an international attendance of more than 1000 skin scientists from around the globe, the four-day event includes short oral presentations from peer-reviewed abstracts, keynote lectures from international experts, sponsored symposia, satellite programs, educational events as well as an important focus on posters, networking and building collaborations amongst scientists. For more information about this year’s meeting visit our website.