Wound healing is a complex and well-orchestrated process to keep our skin intact. This delicate repair process often gets disrupted in patients with underlying causes. However, the effective wound therapy is lacking due to the limited knowledge of the related molecular pathogenesis. In this study, we performed paired small RNA (miRNA) and long RNA (circular RNA and mRNA) sequencing in human tissue samples, including matched skin and acute wounds collected at each healing stage (Day 1 and Day 7) and chronic non-healing venous ulcers (VU). Based on these transcriptomic data, we developed two user-friendly compendia deployed in our server (miRNA Shiny app: https://www.xulandenlab.com/humanwounds-mirna-mrna, circRNA Shiny app: https://www.xulandenlab.com/humanwounds-circrna), which will be an open, comprehensive resources to broadly aid wound healing research. In these resources, we visualized easily the single expression and changed expression patterns of miRNA, circRNA and mRNA acoross different conditions. In addition, we also identified several circRNAs and miRNAs, which can perturb the cellular processes of inflammation, migration, and proliferation in wound healing. Findings in this study will provide a rich resource for developing effective targets for treating no-healing chronic wounds.

ESDR599 - The compendium of profiling coding and non-coding RNAs in human skin wounds

The scientific meeting of the European Society for Dermatological Research (ESDR) is held yearly, usually in September. With an international attendance of more than 1000 skin scientists from around the globe, the four-day event includes short oral presentations from peer-reviewed abstracts, keynote lectures from international experts, sponsored symposia, satellite programs, educational events as well as an important focus on posters, networking and building collaborations amongst scientists. For more information about this year’s meeting visit our website.