Psoriasis is a complex disease and recently the intestinal microbiome has gained interest in psoriazis pathogenesis, therefore microbiome restoration becomes a promising preventive/therapy strategy. In this pre-clinical study using a mice model of psoriatic dermatitis (Ps), we have tested the proof-of-concept that IgY raised against pathological human bacteria resistant to antibiotics can alleviate psoriatic lesions and restore immune parameters. Standard Ps murine model was performed using the imiquimod-based cream application for 6 consecutive days. Four groups of C57BL/6 mice, were constituted: Ps group, IgY treated Ps group, naturally remitted Ps group and control group. Besides clinical evaluation, splenomegaly and histopathological assessment, cellular immune parameters were monitored using flow cytometry. We have quantified T lymphocytes (CD3ε+), T-helper (CD4+CD8-) and T-suppressor/cytotoxic (CD8a+CD4-), B (CD3ε-CD19+) and NK cells (CD3ε-NK1.1+) from peripheral blood (PB) and spleen cell suspensions (SCS).
Splenomegaly, a characteristic of Ps mice, was completely remitted after IgY treatment. For Ps group, the main changes observed in both PB and SCS were significant lower percentages of T-CD4+ (p<0.05) and B cells (p<0.05), while T-CD8a+ (p<0.05) and NK1.1+ cells (p<0.05 in PB) were significantly increased as compared to controls. After IgY treatment, for all investigated parameters a tendency of normalization of the values was observed.
Improved clinical evolution of the induced Ps along with the restoration of immune cells parameters were obtained when orally IgY was administered. We pin-point that IgY specific compound can be used as a possible pre-biotic alternative adjuvant in Ps. As IgY preparation can be raised against individualized microbiome, therefore using this compound can open the personalized medicine domain in Ps.

LB001 - Evaluation of immune parameters in a psoriatic mouse model addressing gut microbiota

The scientific meeting of the European Society for Dermatological Research (ESDR) is held yearly, usually in September. With an international attendance of more than 1000 skin scientists from around the globe, the four-day event includes short oral presentations from peer-reviewed abstracts, keynote lectures from international experts, sponsored symposia, satellite programs, educational events as well as an important focus on posters, networking and building collaborations amongst scientists. For more information about this year’s meeting visit our website.