The water extractable (WE) and alkali extractable (AE) Hemicellulose A (Hemi. A) Hemicellulose B (Hemi. B), oligosaccharides (Oligo) and cellulose-rich arabinoxylan (CAX) fractions were isolated from bran of four wheat varieties and characterized. Wheat bran from all varieties were rich in protein content (12.45 - 16.97%). The yield of Hemi. B fraction varied from 1.2 to 4.9 % in all wheat verities. The sugar composition of all fractions was estimated using high-performance anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD), in which the Hemi. B fraction showed a typical arabinoxylan structure containing arabinose and xylose as main sugars with small amount of other sugars such as rhamnose, galactose, glucose, galacturonic acid and glucuronic acid. The study of emulsion stability of the Hemi. B fraction was performed, in which the Hemi. B from C-306 wheat bran variety exhibited a good ability to form a very stable emulsion. The yield of cellulose rich arabinoxylan varied from 9.5 to 13.0 %, and they all had a high-water holding capacity (13.3 to 16.13 g/g water/CAX).

Fractionation and characterization of functional components of wheat brans

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