The separation of small molecules from complex samples often poses the ultimate challenge to any analytical method development process, whether for targeted or non-targeted analysis.<br/>Moreover, trends in the development of new microseparation techniques for extraction of small molecules have recently shifted toward greener and faster approaches, guaranteeing minimal consumption of organic solvents, promoting the production of reusable extraction devices and enhancement of analysis throughput through automated systems. Solid Phase Microextraction is an alternative extraction technique that complies with all the features above and provides simultaneous extraction/enrichment of the targeted analytes.<br/>Applying microextraction to environmental and bioanalysis provides unique opportunities such as minimal sample handling, on-site and in-vivo sampling. This work describes the development of innovative strategies for quantitative analysis of small molecules, namely environmental pollutants and xenobiotics, in complex samples using microextraction approaches that guarantee enhanced preconcentration. Microextraction, at the same time, drastically minimizes laboratory waste production, thus providing a more sustainable solution for chemical analysis.

Sustainable microseparation methodologies for small molecules extraction in complex systems

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