Post-transcriptional modifications play vital role in maintaining structure, stability and function of RNA molecules. Absence or alteration of modification levels have been implicated in human diseases. UVA exposure has been shown to cause oxidative damage to the post-transcriptional modifications (PTMs) in RNA following activation of cellular photosensitizers and generation of reactive oxygen species. While the levels of PTMs in tRNA have been shown to go down, it is not clear about the nature of conversion products and their contribution to the structure and function of RNA molecules. By adopting an untargeted liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) based analytical approach, we identify the oxidative degradation products of specific nucleoside modifications and conditions of their production. We would discuss the ongoing work on the effects of such changes to tRNA structure and implications of such changes on translation function during gene expression process.

Detection of photooxidative products of tRNA modifications by liquid-chromatography coupled with tandem mass-spectrometry

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ACS Fall 2022

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