Uracil is a common type of lesion in DNA that arises from either spontaneous deamination of cytosine or errors in the replication process. DNA containing uracil is excised from the genome in the base excision repair process by uracil DNA-glycosylase (UDG). It is hypothesized that UDG activity is dependent on the intrinsic deformability of the base steps neighboring the uracil. A diverse set of uracil-damaged DNA sequences have been studied using molecular dynamics (MD) simulations. Global and local flexibilities have been investigated and compared to rigidity measures from florescence and NMR proton imino exchange experiments. Simulation measures correlate with the experimental findings and measured repair efficiencies and show the impact of sequence context on uracil flexibility.

Sequence effects on the flexibility of uracil damaged DNA

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.