The efficient, accurate, and reliable quantification of cannabinoids has increasingly become a burgeoning field of research. Many are interested in these compounds due to their well-known therapeutic properties. In addition to the stringent production and labeling regulations that accompany the legalization of cannabis and cannabis products throughout the United States. Undergraduate programs have started to incorporate courses and hands-on research specific to cannabis chemistry. One limiting factor includes the expense and expertise characteristic of the separation and quantification of cannabinoids. Typically, HPLC equipped with a UV detector is utilized to analyze both acidic and neutral forms of cannabinoids. Often, these systems require significant upfront expenditures and a designated analyst. Herein, we highlight the use of Lucidity’s new HPLC-UV system, the miniLC, to separate and quantify a mixture of 16 cannabinoids. Quantification is performed using an internal standard and pure reference compounds for the construction of calibration curves. All calibration curves displayed R<sup>2</sup> values &gt;0.999.  Advantageously, the miniLC boasts a small footprint, allowing for easy movement and point of need analysis.  The system is also affordable and equipped with software designed to be accessible to all users, allowing for easy integration into curriculum. The miniLC has proved to be a valuable analytical tool, opening this form of analysis to educational laboratories and to scientists earlier in their academic careers.  The miniLC is an excellent option for undergraduate laboratories seeking a compact, simple, and lower cost HPLC-UV system.

Cannabinoid separation: A new HPLC system suitable for cannabis research in undergraduate laboratories

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.