Scanning electron microscopy (SEM) has been an effective way of imaging biological samples for decades. There are numerous well-established methods for preparing samples for examination via SEM. Many of these methods rely on toxic metals such as osmium or toxic organic reagents. These reagents are needed in order to ensure the cells within the tissue remain intact as SEM data are collected under vacuum and without proper treatment the integrity of the sample would be destroyed. This project will focus on other less toxic reagents that can be used to fix biological samples and explore the different drying methods used such as super critical carbon dioxide and hexamethyldisilazane (HMDS). A comparison of the different fixing agents as well as drying agents will be present.

Examining fixing agents for scanning electron microscopy on biological samples

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.