Volatile organic compounds (VOCs) in urine and other biological sample types have been demonstrated as biomarkers for breast cancer and many other diseases. Our group has investigated breast cancer progression through analyzing urinary VOCs over the course of three weeks using murine models. This analysis identified a panel of urinary VOCs with the ability to classify cancer and monitor tumor progression (&gt; 90% cross validated accuracy). However, an unanswered question is whether urinary VOCs be utilized to monitor the efficacy of antitumor treatments over the course of time. Herein, subsets of tumor-bearing mice were treated with Pitavastatin at high (8 mg/kg) and low concentrations (4 mg/kg), and their urine was collected and analyzed through solid phase microextraction (SPME) coupled to gas chromatography-mass spectrometry (GC-MS). VOC profiles from mice treated with Pitavastatin were compared to the previously analyzed healthy controls and tumor-bearing untreated mice through chemometric analyses. Previous investigations using X-ray and microCT indicated that Pitavastatin administered at 8 mg/kg had a protective role against mammary tumors, while 4 mg/kg treatments did not inhibit tumor-induced damages. Univariate and multivariate statistical analyses of urinary VOCs showed that mice treated with Pitavastatin at high concentrations were significantly different from tumor-bearing untreated mice (in the direction of healthy controls), and mice treated with low concentrations demonstrated significant differences relative to healthy control mice (in the direction of tumor-bearing untreated mice) over the course of three weeks. These results show that urinary VOCs can noninvasively predict the efficacy of Pitavastatin treatments over time.

Chemometric analysis of volatile organic compounds in mouse urine accurately monitors the efficacy of pitavastatin treatments on mammary tumor progression over time

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ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.