<br/>Infectious disease outbreaks are an ever-growing threat to society due to mounting environmental pressures increasing interactions between people and zoonotic disease reservoirs. Engineering precise, rapid, and deployable diagnostics will be critical at identifying these threats to prevent future outbreaks from occurring, or if an outbreak does occur, at containing the disease to minimize public health and economic burden. Cas12 is an emerging biosensor that detects genetic biomarkers and has potential to enable such diagnostics. However, first-generation Cas12 biosensors are insensitive and require DNA amplification prior to detection. To address this limitation, we engineered autocatalytic CRISPR-Cas biosensors to improve speed-of-detection and assay sensitivity. Herein, I will describe these developments and highlight the capabilities of auto-catalytic CRISPR-Cas biosensors.

Auto-catalytic CRISPR-Cas biosensors for rapid disease detection

Fall 2022 abstracts, posters, and presentations will launch in mid-September.

ACS Fall 2022

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.