Apoptosis, the process of programmed cell death, plays an important role in cell growth and proliferation. It is of great significance to detect apoptosis-related substances and monitor the apoptosis process for promoting the development of medical research. Fluorescence imaging analysis based on resonance energy transfer (RET) can realize intuitive and sensitive apoptosis analysis. However, traditional single donor-single receptor RET designs are often limited by high background signals due to low RET efficiency. Graphene oxide (GO) is not only capable of absorbing single stranded DNA, but also an effective fluorescence quenching agent. DNA/GO nanodevices constructed by direct physical adsorption of single stranded DNA with GO have been used in apoptosis imaging analysis. However, the interaction between single stranded DNA and GO in such DNA/GO nanodevices is susceptible to competition from biomolecules, resulting in false positive signals. Here, a one-donor-two-receptor DNA/GO nanodevices was constructed, which greatly improved the RET efficiency. In addition, the double-stranded DNA was stably adsorbed on the GO by poly-A, and the false positive signals was reduced by using the FRET between the donor and BHQ. The DNA/GO nanodevice was used for simultaneous monitoring of apoptosis-related miR-630 and miR-21 with low background, high accuracy and high selectivity. Furthermore, on the basis of one-donor-two-receptor DNA/GO nanodevice design, a sequential resonance energy transfer-based GO-DNA nanodevice was constructed for precise regulation and process monitoring of apoptosis.

Resonance energy transfer-based DNA/graphene oxide nanodevice for apoptosis imaging analysis

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ACS Fall 2022

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