An ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry (UHPLC-ESI/MS/MS) method was developed for quantification of cannabigerol (CBG) among twelve cannabinoids in hemp oil. The quantification was achieved using internal standard calibration between 0.01 and 12.5 mg/mL with CBG-d<sup>9</sup> as internal standard. The limits of quantitation (LOQ) was determined to be 0.008% CBG in hemp oil. To recover CBG, hemp oil was combined with methanol to prepare a 25 mg/mL mixture. After ultrasonication, centrifugation and filtration, the extract was serially diluted to 125 mg/mL containing 0.5 mg/mL CBG-d<sup>9</sup>. The measurement precision in triplicate was 9.2%. The method is not interfered by other cannabinoids present in hemp oil.

Quantification of cannabigerol in hemp oil by ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry

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